1、 Summary

In the past20During the year,RIARadioimmunoassay has been largely replaced by non radiometric analysis methods, such asELISAEnzyme immunoassay,FIAFluorescence immunoassay orLIAFluorescent enzyme immunoassay method.ELISAEnzyme immunoassay has become a common choice for immunoassays due to its relatively low cost and open structural platform.

In recent years, with the development of horseradish peroxidase and phosphatase luminescent materials, they have the ability to directly replace chromogenic substrates, resulting in substantial increases in sensitivity, speed, and dynamic range.

There is another important factor that cannot be ignored: the highly sensitive luminescent bacterial biotoxicity analyzer developed and produced by technology guided enterprises has a special design specifically for immune analysis, which can bring excellent analytical performance; Simultaneously possessing global service capabilities, and more importantly, reflecting good cost-effectiveness.

The only one who can currently complete this challenge isAmercia UniBest Group Corp.Designed and produced by the companySafeLight ProHigh sensitivity microplate luminescence analyzer. Our company has successfully manufactured thousands of luminescent bacterial biotoxicity meters for medical diagnosis and research.

2、 Product Introduction

Amercia UniBest Group Corp.The company's experience in producing thousands of photon counters ensures the sensitivity and stability of the detectors. The advantage of true photon counting is that it does not require setting user parameters, ensuring that the conditions for each measurement are the same throughout the lifespan of the instrument. The design of the optical system can achieve optimized spatial interference (which can be reduced to...)10^-6Related to the type of orifice plate.

measurable96Orifice plate8Perforated plate and12Hole strip board.

Accurate measurement within the instrumentxandyDirectional drive ensures accurate positioning for different detector orifice plates.

3、 Product evaluation

1Evaluation of reagent manufacturers

SafeLight ProThe instrument is known for its sensitivity, making it suitable for clinical testing with lower reagent consumption. High quality standardization processes can be widely selected to ensure accuracy in analysis and measurement.

2Researcher evaluation

A popular instrument with good performance and small size for genes and adenosine triphosphate（ATP）Enzyme body（caspase）And kinase（kinase）The application of report analysis is an ideal choice.

4、 Product Features

1、 software

The designed software is suitable for daily clinical experiments and can provide detection schemes for all types of immunoassays.

2Unlimited number of detection programs

Can store an unlimited number of editing programs. The program type includes immune type（ILMA）Competition type（LIA）, Yin/Positive qualitative type and original data type.

3Automatic performance testing

Perform automatic performance testing using optional test boards.

4Flexible definition

Flexibly define the orifice plate to be measured.

5selection order

You can choose horizontal or vertical strip analysis.

6multiple analysis

Each orifice plate can have multiple analyses.

7full support

Fully support matching reagent kit numbers.

8Save analysis results

Allow offline reassessment.

9oscillating

Different amplitudes and velocities can be selected for oscillation.

10Intuitive and simple menu

The intuitive driver guide menu can guide users to complete all instrument parameter settings and determine analysis methods. Users only need to follow the on-screen menu prompts to enter the necessary parameters for a specific analysis method. Clear and easy to understand dialogue boxes makeSafeLight ProThe device becomes an interface friendly and easy-to-use instrument.

After setting the parameters of the analysis method, the program can be saved and called, modified, and measured from time to time. In daily operations, users can select the desired analysis program, place it directly on the orifice plate, and then start measuring.

11Curve fitting

Curve fitting provides various formats, allowing for standard, allB0andNSBMultiple copies of (empty).

12High performance curve fitting

Complex mathematical fitting curves includelogit-logperhapslog-logCurve conversion. Based on the third-order curve function and following the curve fitting algorithm, automatic smoothing, manual smoothing, or linear regression have been optimized.

13Save the standard curve

The saved standard curve can be manually entered data（MasterCurve), or reference the measured values of the curve, or directly save the new curve used.

14Two point calibration

Use Save Curve orMasterPerform two-point calibration on the curve.

5、 Application

1Luminescence immunoassay method

Specially designedSafeLight ProLuminescence immunoassay analyzer is an ideal instrument for various types of immunoassay. Replacing the chromogenic substrates of horseradish peroxidase or phosphatase with luminescent substrates can improve accuracy100Twice. The software with integrated curve adjustment function makes the operation easier, and the powerful data analysis and processing capabilities make the instrument's performance more outstanding.

2Genetic analysis report

All luminescent type gene analysis reports can be usedSafeLight ProInstrument measurement. In the basic research of gene and drug development, luciferaseβ-Galactosidaseβ-grape[grapes]Glycosidase has become the standard method for obtaining highly sensitive detection.

Even for dual luminescence type analysis, such as dual luciferase reporter analysis, reagents can be manually added and usedSafeLight ProInstrument measurement. These analytical methods have become popular analytical tools because they can achieve internal control of cell transfection efficiency and reach the technical level of large-scale gene expression.

3Adenotriphosphate（ATP）Testing

Used for adenosine triphosphate（ATP）The analysis of glow type detection, due to the generation of fluorescence, can be usedSafeLight ProInstrument measurement. Because all living cells have adenosine triphosphate（ATP）So cell proliferation analysis or bacterial detection analysis can also be measured using this instrument.

4Enzyme body（caspase）And kinase（kinase）Analysis

cysteine-The enzymatic activity assay of aspartic acid peptidase composition is a key method for studying cell apoptosis. This analysis method designs special peptide substrates for the separated enzyme bodies3、7、8、9When the enzyme body appears, it indicates that the cell is in a peptide state.

This analytical technique is achieved by interpreting the luminescent material through the release of luciferin, which is the enzyme reactant of the luciferase reaction that produces light.

The action of luciferase can be used for the detection of kinases. Kinases can alter the activity of specific proteins and are widely used in signal transduction and control of complex processes in cells.

Its enormous diversity and role in signal transduction make it a key focus in research and drug design

Required indicators.

6、 Technical specifications and ordering information

（1） TheTechnical description

Detector: Low noise single photon counting photomultiplier tube

sensitivity ：0.03 mIU/L TSH20 amol ATPTriphosphate glycoside~ 7.5zmolFirefly luciferase,

Dynamic range:> 6Rank level,

Inter hole interference: By reducing inter hole interference through design, lower inter hole interference can be achieved<10^-6，

Orifice plate format:96Opaque orifice plates and strips,

External dimensions: (length)xwidexHigh)85.48 x 127.76 x 14mm，

Interface:USB，PC

operating system ：Windows.

PCConfiguration requirements: withUSBDesktop or laptop computers with interfaces.

Power Supply:110-240V，50/60Hz，30VAExternal self variable range power adapter, standard specifications:CE，UL，CSA，

Temperature range: Storage0-40℃, Operation15-35℃

Humidity:10-85%Non spotlight, size:300 x 400 x 200mm(Wide)xlongxHigh)

Weight:6 kg，

Measurement time:0.1arrive600second

Measurement: Horizontal or Vertical

Delay: Maximum up to600second

Oscillation: Variable amplitude and speed.

（2） Software functions

1、 Create Parameter Input Wizard

2、 Orifice layout input（NSB/EmptyB0Standards, controls) and replication (up to10）

3、 Raw data analysis (such as gene report analysis)

4、 yin/Yang qualitative measurement

5、 Immune type（ILMA）And competitive type（LIA）Immunoassay

6、 Number line conversion selection:log/log，logit/log

7、 Through cubic curve functions, linear regression, or4PLPerform standard curve calculation

8、 Fully standardized (upper limit)10A standard)

9、 MasterCurve (a universal standard curve generated by calibration adjustment method)

10Reference curve (the measured curve becomes...)MasterCurve)

11The use of new measurement curves

12Compliant withGLPGood drug experimental research standards, supporting reagent matching equipment numbering

（3） Order information

SafeLight ProMicroporous Plate Luminescence Analyzer

used forSafeLight ProThe luminous test version

Amercia UniBest Group Corp.The company reserves the right to implement technical improvements and design changes without prior notice.

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